BS EN ISO 10504:2015 pdf free.Starch derivatives — Determination of the composition of glucose syrups, fructose syrups and hydrogenated glucose syrups — Method using high-performance liquid chromatography (ISO 10504:2013).
BS EN ISO 10504 describes a high-performance liquid chromatographic (HPLC) method for measuring the composition of dextrose solutions, glucose syrups, fructose-containing syrups, hydrogenated glucose syrups, sorbitol, mannitol and maltitol. The constituents are mainly glucose, maltose, maltotriose, fructose, sorbitol, mannitol, maltitol and malto-ol igosaccharides.
The use of a column packed with cation-exchange resin is essential.
2 Normative references
The following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 3696:1987, Water for analytical laboratory use — Specification and test methods
ISO 5381:1983, Starch hydrolysis products — Determination of water content — Modified Karl Fischer method
3 Principle
Saccharide components are separated using high-performance liquid chromatography. Separation is achieved using a cation-exchange column with water as the eluent. The eluted components are detected by means of a differential refractometer, and quantified using an electronic integrator.
4 Reagents
All reagents used shall be of recognized analytical reagent grade.
4.1 Special distilled water.
The water used may be double-distilled of quality grade 1 in accordance with ISO 3696. The most suitable is demineralized water, which prevents contamination of the ion-exchange resin.
The water should be filtered by passage through a 0,22 tm filter. Also, it should be degassed by treatment under vacuum, or by use of an in-line degassing unit. The water should be maintained under an inert atmosphere, and preferably at 70 °C to inhibit microbial growth.
NOTE Some commercial water-purification devices produce water which is both filtered and degassed.
4.2 Primary standard solutions.
Prepare solutions (see Annex A) containing 10 % (or less) dry matter, according to the sensitivity of the refractometer, with compositions as close as possible to that of the samples to be analysed.BS EN ISO 10504 pdf download.