ISO 16373-2-2014 pdf free download.Textiles – Dyestuffs – Part 2: General method for the determination of extractable dyestuffs including allergenic and carcinogenic dyestuffs (method using pyridine-water).
The following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies
ISO 3696, Water for analytical laboratory use — Specification and test methods
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1 allergenic dyestuff dyestuff which may cause an allergic skin reaction
3.2 carcinogenic dyestuff dyestuff which is classified as carcinogenic substance
Note 1 to entry: Harmonized classification according to the Globally harmonized system of classification and labelling of chemicals (GHS) ,IZI incorporated in EU Regulation 1272/2008 (CLP)i1i.
4 Principle
A coloured test specimen is selected from the textile article and extracted with pyridine/water at
100 °C. The extract is analysed by liquid chromatography/diode array detection (LC/DAD) and/or by
liquid chromatography/mass spectrometry (LC/MS).
5 Safety precautions
WARNING —The substances listed in Tables Ad. Bi and 82 are classified as substances known to be or suspected to be human allergens or carcinogens.
Ensure that any handling and disposal of these substances Is In strict accordance with the appropriate national health and safety regulations.
It is the user’s responsibility to use safe and proper techniques in handling materials in this test method. Consult manufacturers for specific details such as material safety data sheets and other recommendations.
Good laboratory practice should be followed. Wear safety glasses in all laboratory areas and a single use dust respirator while handling powder dyestuffs.
Attention is drawn to any national and local safety regulations.
6 Reagents
Unless otherwise specified, analytical grade chemicals shall be used.
6.1 Pyridine.
6.2 Acetonitrlle, chromatographic grade.
6.3 Ammonium acetate.
6.4 Tetrabutylanimonium dihydrogen phosphate.
6.5 DeIonized water, grade 3 in accordance with ISO 3696.
6.6 Pyridine/water (1:1), prepared by mixing 500 ml pyridine (6.1) and 500 ml water (6.5).
Keep the solution in a brown glass bottle.
6.7 Individual stock solutions, prepared in pyridine-water 1:1, of all reference substances listed in Annexes A and B
It is recommended that reference substances (including those listed in Annexes A and B) of the highest purity grade available on the market be used. The given purity has to be considered for the calculation (see Clause 9).ISO 16373-2 pdf download.
ISO 16373-2-2014 pdf free download
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